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China (Mainland)
Since 2012-06
Shanghai Crystal day Biotech Co., Ltd.
Address:Room 1306,Building 1,Lane 60,Zhongyuan Building,Central Plains Road, Yangpu District,Shanghai, China
Contact Person:Mr.zoe

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Rat 3-Nitrotyrosine,3-NT ELISA Kit (E0019Ra)

Min Order Quantity:1 Box/Boxes
FOB Price:368-578(USD) per Box
Payment Terms :T/T
Production Capacity :500/Box/Boxes per Month

【Intended Use】
This kit is used to assay the 3-Nitrotyrosine,3-NT  in the sample of human’s serum, blood plasma, and other related tissue Liquid.
【Specimen requirements】 
1. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
2. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
3. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
4.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation ofHydrothorax and cerebrospinal fluid Reference to it.
6.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
7.Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
【Calculate】
Take the standard density as the horizontal,the OD value for the vertical,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve (the result is the sample density)。or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the 
equation, calculate the sample density.
 

Contact Details

Company Name: Shanghai Crystal day Biotech Co., Ltd.
Company Address: Room 1306,Building 1,Lane 60,Zhongyuan Building,Central Plains Road, Yangpu District,Shanghai, China
Country/Region: China (Mainland)
Zip/Postal Code: 200438
Phone Number: 86-21-51872883
Fax Number: 86-21-65578917
Homepage: http://www.bt-laboratory.com/
Contact Person (Department): Mr.zoe